Fig. 8.

EX-527 reversed the protective effects of NMN against the oxidative stress and inflammatory responses. (A–F) Expression levels and the relative quantification of SIRT1 and PGC-1α, and the acetylation level of lysine in hippocampus. (G–I) Phosphorylation and the relative quantification of P38 and P65 in hippocampus. (J) Representative images of ROS immunostaining in hippocampus. Scale bar = 20 μm. (K) The activity of SOD in hippocampus. (L) MDA content in hippocampus. (M) TNF-α in hippocampus. (N) IL-6 in hippocampus. (O) IL-1β in hippocampus. (P) Plasma TNF-α. (Q) Plasma IL-6. (R) Plasma IL-1β. Tukey post-hoc test following one-way ANOVA was used. The values were presented as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4 or 6 for each group).