Figure 5.
Effect of BEECs pretreatment with HA on sperm- induced inflammation. (A–C) BEECs monolayers were incubated without- or with HA (at 0.1, 1, or 10 µg/mL) for 2 h prior to the co-culture with 106 sperm/mL for 3 h. Then, mRNA expressions of TLR2, TNFA, IL-1B, IL-8, and PGES were quantified in BEECs via a real-time PCR. Data are presented as mean ± SEM of 3 independent experiments using epithelial cells from 3 different uteri (3 wells per treatment per experiment). Different letters denote a significant variance (P<0.05) between the different groups when compared with One-way ANOVA followed by Tukey’s multiple comparisons test.