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. 2023 May 10;14:1138676. doi: 10.3389/fendo.2023.1138676

Figure 1.

Figure 1

Effect of U60E on cell viability of pericytes and human retinal microvascular endothelial cells (HRMECs). Pericytes (A) and HRMECs (B) were treated with U60E for 72 h at indicated doses. The cell viability was determined by the MTT assay. The bar graph represents the means ± standard deviation (SD) (n = 5). Pericytes (C) and HRMECs (D) were treated with U60E for 72 h at indicated doses under the conditions of high glucose (HG; 30 mM glucose). Normal glucose (NG; 5 mM glucose) and high mannitol (HM; 5 mM glucose and 25 mM mannitol) were used as controls. The cell viability was determined by the MTT assay. The bar graph represents the means ± SD (n = 5). *P < 0.05. Pericytes (E) and HRMECs (F) were treated with U60E (20 μg/ml) for 72 h under the conditions of HG, with or without tumor necrosis factor α (TNF-α) (100 ng/ml). The cell viability was determined by the MTT assay. The bar graph represents the means ± SD (n = 5). No significance (n.s.) indicates P > 0.05, *P < 0.05.