FIG. 3.

EBER expression is maintained within tumors induced by EBV⁻/EBER⁺ BL cells. EBER expression within each EBV-negative 3F2- and A.2-derived cell line (cl) that expressed EBERs at 41 to 127% of EBV-positive (A.15) Akata cells (Fig. 1) was compared to EBER expression within a respective tumor (t) by Northern blot analysis. Blots were also probed for GAPDH mRNA as a control for RNA loading and integrity. The level of EBER expression, normalized to GAPDH, is presented below each lane. When blots were sequentially stripped and rehybridized to EBER-specific probes, the ratio of EBER-1 to EBER-2 expression had not changed in the tumors relative to that in the parental cell lines (data not shown).