FIGURE 4.

Cell-based device promotes excisional wound healing and bone regeneration in a calvarial defect. (A) Excisional wound was made in C57BL/6 mouse and fixed using a wound splint. The cell-based device with control cells (n = 4) or Combo 8 (n = 4) was inserted in the middle of the wound. After 7 days, healing was evaluated, and histological analysis was conducted. Representative photos of wounds at the beginning and after 7 days are shown. Histological analysis of wounds stained with H&E and Masson’s trichrome (TRI) is presented (scale bar represents 500 µm). (B) Schematic representation of the experimental setup. (C) Quantification of surface area measurements of defects based on micro-CT scans was performed. The average surface area per each animal is shown, and the mean ± SEM per condition are presented (n control = 6 animals, n Combo 5 = 5 animals), statistical analysis with a two-tailed t-test (*p < 0.05, **p < 0.01, ***p < 0.001). (D) Representative micro-CT scans of the calvarial defects after 2 months and histological analysis. Magnified insets are shown on the right. Arrows indicate the margins of the defect.