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. 2023 May 2;8:76. Originally published 2023 Feb 14. [Version 2] doi: 10.12688/wellcomeopenres.18937.2

Figure 5. Hyaluronan binding by soluble Transmembrane protein 2 (sTMEM2).

Figure 5.

( A) Size exclusion chromatography of sTMEM2 in the absence or presence of unlabelled HMW-HA. To avoid overlap of the two traces, the red trace has been shifted by +10 mAU. No sTMEM2 protein is associated with HMW-HA eluting in the void volume of the column (arrow). ( B) Binding of sTMEM2 and the aggrecan G1-link protein complex (HA-binding protein) to HA oligomers of different length (dp, degree of polymerisation). The experiment was done using a neoglycolipid-based microarray 18, 19 . Data (fluorescence intensities of HA probes arrayed at 5 fmol/spot level) are presented as mean values ± range for n = 2 duplicate spots on the array. The raw measurements are available in Underlying data.