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. 2023 May 1;16:e00223. doi: 10.1016/j.mec.2023.e00223

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Mutational analysis of evolved isolates. A) Sankey diagram showing the relation between gene function, gene name, and the strain backgrounds. B) Heat map of converged mutated genes across independent TALE and ALE experiments for different backgrounds. Numbers indicate the number of evolved isolates in which mutations in the given genes or genetic regions were observed. In the cumulative TALE column, the total number of evolved isolates in which mutations were observed for all TALE experiments are shown. *Genes associated with the flagellar structure are pooled, these include fliG (TALE#9), fliR (TALE#9, #10, ALE #28), fliH (TALE #12, ALE #21), fliF (TALE #15, ALE #23), fliL (TALE #13), fliK (TALE #16, ALE #27), fliY, (TALE #17), fliZ (TALE #18, ALE #24), fliP (TALE #19), flhA (ALE #26).