Fig. 5.

A) Structure of the CodY protein (PDB ID:5loe). Mutated amino acids and their side-chains are indicated in red and the HTH DNA-binding domain is colored pink. Structure was visualized using PyMOL (Schrödinger LLC). Numbers in the table indicate the number of evolved isolates carrying mutations in the specific residue. B) Pictures of end isolates TALE#5 I17 and TALE#9 I13 after 24h growth in cal-18 medium. The dark brown patches of biofilm were only present for TALE#5 I17. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)