Fig. 4.

Egr1 knockdown alters transcriptome in hepatocytes.

(A–G) Primary murine hepatocytes were transfected with indicated siRNAs followed by treatment with PA (0.2 mM). RNA-seq was performed and analysed as described in the Materials and methods section. (A) PCA plot. (B) Volcano plot. (C) GO analysis. (D) KEGG analysis. (E) GSEA. (F) Heatmap of differentially expressed genes. (G) HOMER analysis. BCL6, B-cell lymphoma 6; Egr1, early growth response 1; ES, enrichment score; FAO, fatty acid oxidation; FDR, false discovery rate; GO, gene ontology; GSEA, gene set enrichment analysis; HOMER, hypergeometric optimisation of motif enrichment; IR, insulin resistance; KEGG, Kyoto Encyclopedia of Genes and Genomes; PA, palmitate; PC1, principal component 1; PC2, principal component 2; PCA, principal component analysis; PPARα, peroxisome proliferator-activated receptor α; RAR: retinoic acid receptor; RNA-seq, RNA sequencing; RXR, retinoic X receptor; SCR, scrambled siRNAs; siEgr1, Egr1 siRNA; siRNA, small interfering RNA; TCF7, transcription factor 7.