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. 2023 May 24;22:122. doi: 10.1186/s12933-023-01842-3

Table 4.

Anti-diabetic, anti-inflammatory/anti-aggregation drugs impacting on ncRNAs

Antidiabetic drugs and ncRNAs
Reference Study Population/experimental system Treatment Main findings
[333] Mouse liver Mice treated with metformin Metformin induced the expression of miR-20a-5p, miR-34a-5p, miR-130a-3p, miR-106b-5p, miR-125b-3p, and let-7c
[334] BJ-1s human neonatal foreskin fibroblasts Cells treated with metformin at different passages Metformin induced the expression of miR-200a, miR-141, miR-429 and of miR-205 in senescent BJ-1s cells
[335] Human umbilical vein ECs Cells treated with metformin at different passages Metformin induced the expression of miR-100-5p, miR-125b-5p, miR-654-3p, miR-217 and miR-216a-3p/5p in senescent ECs
[270] Mouse bone marrow-derived endothelial precursor cells Cells treated with palmitic acid and metformin Metformin reverted angiogenesis impairment caused by palmitic acid by attenuating miR-130a/p-AKT axis and increasing PTEN expression
[336] Mouse microvascular ECs Cells exposed to HG and treated with metformin Metformin reduced and increased miR-34a-5p and SIRT1 expression levels, respectively, attenuating HG-induced angiogenesis impairment
[337] Neonatal rat ventricular cells Cells exposed to H2O2 and treated with metformin Metformin reverted H2O2- and ischemia/reperfusion-induced miR-1a-3p expression, reducing cell death
Mouse ischemia/reperfusion Hearts of mice that underwent the I/R injury and treated with metformin
[273] Mouse ischemia/reperfusion Hearts of mice that underwent the I/R injury and treated with metformin Metformin reduced I/R induced-miR-34a-5p expression
H9C2 rat cardiomyocyte cells Oxygen-glucose deprivation/recovery and treatment with metformin Metformin reduced miR-34a-5p levels through decreasing SIRT1-p53 activity
90 ACS (STEMI) patients Metformin 3-months treatment pre-infarction Metformin reduced serum miR-34a levels and CKMB activity and mitigated PCI-induced reperfusion injury
Thoracic aortas of diabetic rats Liraglutide treatment Liraglutide reduced miR-34a-5p and increased the anti-apoptotic protein Bcl2 and SIRT1, contrasting cell death
[280] 25 patients with T2DM Serum from patients with DM treated with liraglutide Liraglutide induced the expression of miR-130a-3p, miR-27b-3p, and miR-210-3p
[338] Mouse MCAO Brain after MCAO and metformin treatment Metformin reduced H19-induced oxidative stress injury
[282] 10 frail old adults with HFpEF and DM Whole blood after 3 months-treatment with empagliflozin or metformin or insulin Empagliflozin specifically reduced miR-21 and miR-92 levels compared to metformin- or insulin-treated HFpEF patients and to controls
Anti-inflammatory/anti-aggregation drugs and non-coding RNAs.
Reference Study Population/experimental system Treatment Main findings
[339] 46 ischemic stroke patients PBMNCs from stroke patients treated with ASA for 10 days ASA induced miR-145-5p levels in stroke patients
VSMCs Cells treated with ASA ASA increased miR-145-5p and decreased CD40 levels, respectively, reducing VSMCs proliferation
[340] Platelets of 12 ASA -treated CVD patients (6 with low and 6 high platelet reactivity) ASA treatment miR-135a-5p and miR-204-5p levels correlated with platelet reactivity
[341] Platelets of 945 acute coronary syndrome patients ASA treatment

-Lower miR-19b‐1‐5p expression was associated to ASA insensitivity and to a higher risk of MACCE

- Low miR-223 was a predictor of responsiveness to antiplatelet therapies

ACS: acute coronary syndrome, ASA: acetylsalicylic acid, Bcl2: B-cell lymphoma 2, CKMB: creatine kinase MB, CVD: cardiovascular disease, DM: diabetes mellitus, ECs: endothelial cells HFpEF: heart failure with preserved ejection fraction, HG: high glucose, MCAO: middle cerebral artery occlusion, I/R: Ischemia-reperfusion, MACCE: major adverse cardiac and cerebrovascular events, PBMNCs: peripheral blood mononuclear cells, PCI: percutaneous coronary intervention, PTEN: phosphatase and tensin homolog, SIRT1: Silent information regulator 1, STEMI: ST-segment elevation myocardial infarction, VSMCs: vascular smooth muscle cells.