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. 2023 May 12;27(6):123. doi: 10.3892/mmr.2023.13011

Figure 1.

Figure 1.

Detection of mouse AKI indexes. (A) Renal injury in the glomeruli of mice in various groups assessed using H&E staining and the protein expression of ACSL4 and GPX4 in the mouse kidney in different groups according assessed using immunohistochemical staining. (B) The fluorescence intensity of ACSL4 or GPX4-expressing region in immunofluorescence (Fig. 1A) was calculated using ImageJ software. (C) BUN and MDA levels of mice in different groups. (D) Iron ion level in the glomeruli of mice in various groups. (E) mRNA expression levels of ACSL4 and GPX4 assessed using reverse transcription-quantitative PCR. Scale bars: H&E, 100 µm; IHC, 50 µm. *P<0.05, **P<0.01 and ***P<0.001. AKI, acute kidney injury; ACSL4, acyl-CoA synthetase long-chain family member 4; BUN, blood urea nitrogen; GPX4, glutathione peroxidase; H&E, hematoxylin and eosin; MDA, malondialdehyde; NC, negative control; RGSH, reduced glutathione; ns, not significant.