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. Author manuscript; available in PMC: 2023 May 24.
Published in final edited form as: Biochem Pharmacol. 2020 Sep 22;182:114226. doi: 10.1016/j.bcp.2020.114226

Fig. 12.

Fig. 12.

Human but not rodent Na/K-ATPase is responsive to cardiac glycosides. (A) A549 cells or mouse embryonic fibroblasts (MEFs) were treated with 500 nM ANTO2 for indicated times and protein expression was examined. (B) The band intensity of each lane in A was quantitated and normalized to that of the control group. Data represent average and standard deviation from 3 replicates. (C) Cell death rate of A549 and MEF cells after 12 h ANTO2 treatment in A was analyzed by trypan blue staining. *P < 0.005. (D) MEF parental cells or those expressing either the wild type human Na/K-ATPase α1 subunit or a rat α1 mutant (D376E) that does not bind cardiac glycoside were treated with 500 nM ANTO2 or not for 12 h, and the dead cell rate was counted by trypan blue assay. Data represent average and standard deviation from 5 replicates. *P < 0.05 was considered significant.