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Cardiac glycoside activates autophagy and apoptosis. (A) U2OS cells were treated with 500 nM ANTO2 for the indicated times, and expression levels of indicated proteins were assessed by specific antibodies. (B) The band intensity of each blot was quantitated by the Image J software and normalized to that in control group. Data represent average and standard deviation from three replicates. *P < 0.05 is considered as significant.
