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Time course effect of ANTO2 on autophagy and apoptosis in A549 cells. (A) A549 cells were treated with indicated concentrations of ANTO2 for 12 h, and protein levels were analyzed by indicated specific antibodies. Arrow indicates cleaved PARP. Short and long exposures for LC3B are shown. (B) The relative protein ratio was quantitated and shown as average and standard deviation from three replicates. *P < 0.005 between control and treated groups.
