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Direct comparison of the tetramer and intracellular cytokine assays. PBMCs derived from individual P8 were either stained with the SL9 tetramer (A) or stimulated with the SL9 peptide for 6 h and stained for the presence of IFN-γ (B). Results are given in terms of the percentage of CD8+ T cells that stained with either the tetramer or IFN-γ.
