TABLE 2.
Comparison of CTL assays
| Volunteer | Result of:
|
||||
|---|---|---|---|---|---|
| IFN-γ ELISPOT assay (total no. of SFC/106 PBMCs [background])
|
SL9 tetramer assay (% of CD8+ T cells responding) | Gag lytic assay (% of bulk lysis specific for rVV Gaga) | |||
| rVV Gag | Gag pool | SL9 peptide | |||
| C1 | 25 (21) | 0 (0) | 0 (0) | NDb | 4 |
| C2 | 9 (5) | 0 (0) | 0 (0) | ND | 0 |
| C3 | 2 (2) | 0 (0) | 14 (0) | ND | 1 |
| C4 | 4 (4) | 1 (0) | 0 (0) | ND | 3 |
| C5 | 0 (0) | 0 (0) | 1 (0) | ND | 4 |
| C6 | 2 (0) | 6 (4) | 4 (4) | ND | ND |
| C7 | 0 (0) | 0 (7) | ND | ND | 1 |
| P1 | 16 (5) | 12 (4) | 100 (4) | 0.11 | 12 |
| P2 | 123 (66) | 6 (3) | 36 (2) | 0.45 | 25 |
| P3 | 186 (8) | 64 (1) | 72 (1) | 0.14 | 45 |
| P4 | 32 (10) | 295 (10) | 139 (10) | 0.24 | 26 |
| P5 | 193 (80) | 20 (2) | 61 (2) | 0.26 | 68 |
| P6 | 240 (62) | 337 (1) | 12 (1) | 0.01 | ND |
| P7 | 24 (7) | 314 (0) | 64 (0) | 0.13 | 60 |
a
Shown at the 50:1 E:T ratio.
b
ND, not done.