Figure 12.

Single-channel analysis of S264A mutant TRESK channels, truncated at amino acid 374, or modified by the replacement of the iCtr with RW361 sequence.A, representative single channel current recordings from two Xenopus oocyte membrane patches in the cell-attached configuration. In the upper recording, the patch contained S264A + Δ374 TRESK channels (more than one), while in the lower recording, a single S264A + RW361 TRESK channel was present. Zero current levels are indicated with horizontal arrows. The potential of the pipette solution was clamped to −100 mV. For more recordings, see Fig. S7. B, statistical analysis of the NP_o values of S264A + Δ374 and S264A + RW361 TRESK channels, as indicated below the graph. The grey circles represent the average NP_o from different membrane patches. ∗p < 0.01, Student’s t test (n = 5, and 6, respectively). C, statistical analysis of the burst duration of S264A + Δ374 and S264A + RW361 TRESK channels, as indicated below the graph. The gray circles represent the average burst duration from different membrane patches. ∗p < 0.03, Student’s t test (n = 5 in both groups). iCtr, intracellular C-terminal region; TRESK, Twik-Related Spinal cord K+ channel.