Figure-2.

Animal reservoir survey using domestic cats and/or dogs. (a) During house-to-house visit, one collection of 1–2 mL blood volume of a domestic cat or dog is obtained by venipuncture at a time close to a peak hour of nocturnally sub-periodic microfilariae of Brugia pahangi. The cat or dog appropriately restrained by field staff is placed in either ventral or lateral recumbency with the forelimb (cephalic vein access) or hindlimb (saphenous vein access). The skin over the collection site that may or may not be clipped with an electric clipper is cleaned with 70% alcohol. (b) Standard microscopic blood examination using capillary tube technique or Giemsa’s stained thick blood smear can be applied under field conditions for screening or diagnosing Brugia spp. parasite infections. [Source: Graphic illustration created by A. Bhumiratana].