Table-2.
Effects of probiotic addition in dog food on the hematological and serum biochemical parameters.
| Parameters1 | Groups2,3 | SEM | p-value | Reference range4 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | ||||
| Hemoglobin (g/dL) | 16.3 | 15.5 | 16.4 | 14.8 | 14.8 | 16.2 | 16.5 | 0.213 | 0.08 | 13.1–20.5 |
| Hematocrit (%) | 48.6 | 46.0 | 49.7 | 45.0 | 44.9 | 48.3 | 50.1 | 0.607 | 0.06 | 37.3–61.7 |
| Red blood cell (106/uL) | 7.72 | 9.49 | 7.73 | 11.1 | 9.49 | 7.79 | 7.96 | 0.715 | 0.82 | 5.65–8.87 |
| MCV (fL) | 62.4 | 62.0 | 64.4 | 63.1 | 63.9 | 62.0 | 62.9 | 0.286 | 0.06 | 61.6–73.5 |
| MCHC (g/dL) | 33.7 | 55.4 | 33.2 | 33.0 | 32.9 | 33.5 | 33.0 | 3.163 | 0.43 | 32.0–37.9 |
| MCH (pg) | 21.0 | 20.9 | 21.4 | 20.8 | 21.0 | 20.8 | 20.7 | 0.101 | 0.17 | 21.2–25.9 |
| White blood cell (103/uL) | 14.9 | 11.5 | 12.7 | 13.9 | 13.1 | 12.7 | 13.0 | 0.299 | 0.30 | 5.05–16.8 |
| Neutrophils (103/uL) | 9.46 | 7.14 | 8.35 | 8.95 | 7.68 | 8.78 | 8.87 | 0.228 | 0.08 | 2.95–11.6 |
| Lymphocytes (103/uL) | 3.56 | 2.75 | 2.73 | 2.61 | 2.99 | 2.23 | 2.39 | 0.121 | 0.15 | 1.05–5.10 |
| Monocytes (103/uL) | 0.65 | 0.53 | 0.53 | 0.60 | 0.68 | 0.51 | 0.54 | 0.024 | 0.06 | 0.16–1.12 |
| Eosinophils (103/uL) | 1.21 | 1.04 | 1.00 | 1.35 | 1.06 | 1.18 | 1.21 | 0.007 | 0.43 | 0.06–1.30 |
| Basophils (103/uL) | 0.05 | 0.03 | 0.03 | 0.03 | 0.04 | 0.03 | 0.04 | 0.003 | 0.45 | 0.00–0.10 |
| Platelets (103/uL) | 137 | 144 | 163 | 208 | 145 | 203 | 163 | 8.944 | 0.06 | 148–484 |
| RDW (fL) | 34.1 | 32.3 | 34.4 | 34.0 | 31.8 | 33.1 | 34.2 | 0.350 | 0.22 | 9.10–19.4 |
| Blood urea nitrogen (mg%) | 11.7 | 12.5 | 12.8 | 12.1 | 12.7 | 12.1 | 11.1 | 0.252 | 0.33 | 10.0–26.0 |
| Creatinine (mg%) | 1.10bc | 1.21cd | 1.16bcd | 1.03ab | 0.95a | 1.25d | 1.05ab | 0.018 | <0.001 | 0.50–1.30 |
| ALT (IU/L) | 44.1 | 30.4 | 33.9 | 25.1 | 30.7 | 31.3 | 34.6 | 2.784 | 0.68 | 6.00–70.0 |
| Total protein (g%) | 6.29 | 6.40 | 6.15 | 6.41 | 6.34 | 6.13 | 8.15 | 0.269 | 0.55 | 5.30–7.80 |
| Albumin (g%) | 3.00 | 2.82 | 3.00 | 2.65 | 2.68 | 2.91 | 3.09 | 0.046 | 0.06 | 2.30–3.20 |
MCV=Mean corpuscular volume, MCHC=Mean corpuscular hemoglobin concentration, MCH=Mean corpuscular hemoglobin, RDW=Red cell distribution width, MPV=Mean platelet volume, ALT=Alanine aminotransferase.
Different superscript letters in the same row represented the statistically significant differences (p<0.05).
Group 1=Control group (none of probiotic supplementation), Group 2=plantarum CM20-8 (TISTR 2676), Group 3=Lactobacillus acidophilus Im10 (TISTR 2734), Group 4=Lactobacillus rhamnosus L12-2 (TISTR 2716), Group 5=Lactobacillus paracasei KT-5 (TISTR 2688), Group 6=Lactobacillus fermentum CM14-8 (TISTR 2720) and Group 7=Mixed of probiotics.
Reference intervals were derived from the Veterinary Diagnostic Laboratory Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand