| formylglycine generating enzyme |
• aldehyde tag
enables variety of chemistries |
• requires a recognition
tag |
| |
• in cellulo modification
by FGE coexpression |
• incomplete conversion results
in heterogeneous population
of nanoparticles |
| |
|
• potential side reactions of aldehyde in complex matrices |
| |
|
• genetic modification
necessary |
| sortase |
•
sortase has a broad nucleophile scope enabling introduction
of variety of conjugate agents (e.g., for labeling and/or tissue targeting) |
• requires a recognition tag |
| |
• modular approach |
• leaves
a scar, e.g., LPET |
| |
•
control at each step |
• more laborious than labeling
during expression |
| biotin ligase |
• biotinylation can be carried out in cellulo by BirA coexpression |
• requires a recognition
tag |
| |
• biotin offers
a purification handle |
|
| |
• flexibility of conjugate agents |
|
