FIG. 2.

Destabilizing the poly(A) hairpin sequence allows completion of self-primed products. The sequence of the poly(A) RNA hairpin was changed so that base pairing between the arms of the hairpin was disrupted. 5′ ³²P-labeled PBS DNA was annealed to the RNA, start solution and RT were added, and the mixture was incubated for 60 min at 37°C, as for Fig. 1A. The reaction was loaded in lane 1, and DNA markers were loaded in lane 2. Sizes of the markers are indicated in bases.