FIG. 3.
Host protein synthesis inhibition in MHV-A59-infected DBT cells. DBT cells were mock infected (M) or infected with MHV-A59 (I) at an MOI of 10. Culture medium was replaced with methionine-cysteine-free medium 30 min prior to each indicated time point. After 30 min of incubation, Tran35S-label was added to the culture medium at a final concentration of 75 μCi/ml. Intracellular proteins were extracted after 30 min of incubation and analyzed by SDS-PAGE (12% gel). Positions of MHV-A59 S, N, and M structural proteins are indicated by arrows.