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MHV-induced 28S rRNA cleavage in RNase L+/+ (A) and RNase L−/− (B) MEF cells. RNase L+/+ (RNL+/+) and RNase L−/− (RNL−/−) MEF cells were mock infected (M) or infected with MHV-A59 (I) at an MOI of 10. Cytoplasmic RNA was extracted at the indicated times and electrophoresed on a 1% denaturing gel. Cleavage of 28S rRNA was examined by Northern blot analysis using 5′-end-labeled probe 1.
