Table 5.
The relative mRNA expression of intestinal epithelial integrity-related genes as affected by dietary CEO supplementation 1
| Item | Con | CEO | SEM | P-value |
|---|---|---|---|---|
| Duodenum | ||||
| ZO-1 | 1.00 | 1.98 | 0.49 | 0.025 |
| Claudin-1 | 1.00 | 1.93 | 0.47 | 0.035 |
| Claudin-5 | 1.00 | 2.04 | 0.52 | 0.014 |
| Occludin | 1.00 | 2.07 | 0.53 | 0.012 |
| E-cadherin | 1.00 | 1.30 | 0.15 | 0.904 |
| Jejunum | ||||
| ZO-1 | 1.00 | 1.10 | 0.05 | 0.911 |
| Claudin-1 | 1.00 | 1.13 | 0.07 | 0.797 |
| Claudin-5 | 1.00 | 1.28 | 0.14 | 0.109 |
| Occludin | 1.00 | 1.93 | 0.46 | < 0.001 |
| E-cadherin | 1.00 | 2.30 | 0.65 | < 0.001 |
| Ileum | ||||
| ZO-1 | 1.00 | 2.57 | 0.79 | < 0.001 |
| Claudin-1 | 1.00 | 3.22 | 1.11 | < 0.001 |
| Claudin-5 | 1.00 | 2.00 | 0.50 | < 0.001 |
| Occludin | 1.00 | 1.33 | 0.17 | 0.366 |
| E-cadherin | 1.00 | 1.88 | 0.44 | < 0.001 |
1 The data were analyzed following the 2−ΔΔCt method, and then the target gene transcript levels were normalized to β-actin