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Primer sequences for qPCR
| Gene | Species | Accession number | Primer sequence | Amplicon length, nt |
|---|---|---|---|---|
| 18S | Rat | NR_046237.1 |
5ʹ-GTAACCCGTTGAACCCCATT-3ʹ 3ʹ-CCATCCAATCGGTAGTAGCG-5ʹ |
151 |
| IL1A | Sheep | NM_001009808.1 |
5ʹ-GTCCATACATGACGGCTGCTA-3ʹ 3ʹ-GGTGTCTCAGGCATCTCCTTAT-5ʹ |
184 |
| IL1B | Sheep | NM_001009465.2 |
5ʹ-CGATGAGCTTCTGTGTGATG-3ʹ 3ʹ-CTGTGAGAGGAGGTGGAGAG-5ʹ |
121 |
| IL6 | Sheep | NM_001009392 |
5ʹ-CGCAAAGGTTATCATCATCC-3ʹ 3ʹ CCCAGGAACTACCACAATCA-5ʹ |
108 |
