FIG. 1.

Isotype-specific ASC to Wa HRV in gnotobiotic pigs inoculated i.n. with two doses of SA11 2/6-VLPs+mLT (■), SA11 2/6-VLPs alone (▩), or Wa-RF 2/6-VLPs alone (▨) or mock inoculated (□) and challenged with virulent Wa HRV. MNC from duodenum, ileum, and MLN (A) and from spleen, PBL, and BM (B) of pigs were collected and tested by ELISPOT assay on PID 21/PCD 0 and on PID 28/PCD 7, respectively. Data represent the mean number of Wa HRV-specific ASC per 5 × 10⁵ MNC for four to five pigs at each time point, except that only two pigs inoculated with Wa-RF 2/6-VLPs were euthanized at PID 28/PCD 7 and no pigs in this group were euthanized before challenge at PID 21/PCD 0. Error bars represent standard error of the mean. ∗, significant difference (Kruskal-Wallis rank sum test, P < 0.05) in ASC numbers between PID 21/PCD 0 and PID 28/PCD 7 for the same isotype in the same group; @, significant difference (Kruskal-Wallis rank sum test, P < 0.05) in ASC numbers between SA11 2/6-VLPs+mLT and SA11 2/6-VLPs groups for the same isotype at the same time point; #, significant difference (Kruskal-Wallis rank sum test, P < 0.05) in ASC numbers compared to mock controls for the same isotype at the same time point.