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. Author manuscript; available in PMC: 2023 May 24.
Published in final edited form as: Methods Mol Biol. 2023;2557:391–416. doi: 10.1007/978-1-0716-2639-9_24

Table 4.

Common transfer vectors for lentivirus and retrovirus

Retrovirus Use Backbone Selection
Retroviral vector for shRNA expression pMKO.1 Puromycin, neomycin
Retroviral expression pBABE Hygromycin, puromycin
Mammalian retroviral gene expression with GFP MSCV-IRES-GFP GFP
Lentivirus Lentiviral expression pWPXL GFP
Lentiviral shRNA expression pLKO.1 Puromycin
Tet-inducible lentiviral shRNA expression Tet-pLKO Puromycin
cDNA expression FUGW Bleomycin
Conditional shRNA expression under the Cre-Lox control pSico Puromycin
cDNA expression pLJM1-EGFP Puromycin

Table 4 describes popular viral transfer vectors that are compatible with retroviral and lentiviral systems for the expression of cDNA or shRNA. Some have features for doxycycline-inducible or Cre-Lox control for conditional expression. Information on the selection marker of the plasmid is also provided