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. Author manuscript; available in PMC: 2023 May 24.
Published in final edited form as: Methods Mol Biol. 2023;2557:391–416. doi: 10.1007/978-1-0716-2639-9_24

Table 5.

Common packaging cell lines

Name Feature
Lenti-X 293 T 6× more virus production than 293FT and 30× more virus than 293 cells
Lenti-Pac 293 Ta Produces high titers
293LTV 293-derived, firm attachment to plates, faster growth rate, produces high titers
293TN Highly transfectable, produces high titers
Platinum Retroviral Packaging 293 T derived, produces high titers, longer stability without drug selection, three versions: ecotropic (Plat-E), amphotropic (Plat-A), and pantropic (Plat-GP)
293RTV Retroviral expression and packaging cell line, faster cell growth, higher retrovirus titer, firmer attachment to plates
Phoenix-AMPHO Amphotropic

Table 5 contains information on commonly used packaging cell lines mostly derived from HEK293 T to achieve higher titers or for longer term virus packing. These cell lines express viral genes (gag, pol) as episomes and therefore are more efficient than other cell lines that require co-transfection of helper constructs to package virus. Some key features related to their viral packaging efficiencies are highlighted in the table for each cell line