Fig. 5. Bacterial thiol-containing metabolites. (a) Heatmap illustrating different levels of thiols in the bacterial lysates. (b) Venn diagram depicting the distribution of common and different features among the three bacterial lysates. (c) Overview of annotated thiols in the lysates of Salmonella (Lysate A), Shigella (Lysate B) and E. coli (Lysate C). (d) MS/MS fragmentation spectrum of the detected conjugate of glutathionylspermidine with annotations of fragments.