Fig. 3.

CBD Treatment Inhibits Macrophage Derived Production of IL-1β Under Inflammatory Conditions. (A) Expression of IL-1β is significantly reduced within in F4/80+ cells selected from the CNS of CBD treated mice in comparison with vehicle treated (n=5). (B) Bone marrow macrophages treated with CBD and stimulated with LPS displayed a significant decrease in IL-1β expression when compared to macrophages treated with DMSO and stimulated with LPS. Bone marrow macrophages treated with CBD and stimulated LPS show no significant change in the expression of IL-1β when compared to bone marrow macrophages treated with CBD alone (n=5, 2-Way ANOVA with Bonferroni’s multiple comparisons test, F (1.000, 4.000) = 28.78. Vehicle + LPS vs CBD + LPS: p=0.0349). (C) Secreted IL-1β protein concentration in media collected from bone marrow macrophages treated with or without CBD under normal and LPS stimulated conditions (n=5, 2-Way ANOVA with Bonferroni’s multiple comparisons test, Vehicle + LPS vs CBD + LPS: p < 0001). (D) Scatter plot demonstrating co-expression of CD45 and IL-1β in CNS infiltrating cells as identified by scRNA Seq data. (E) Scatter plot demonstrating co-expression of CCR2 and IL-1β in CNS infiltrating cells as identified by scRNA Seq data. (F) Representative fluorescent microscopy images of CCR2 expression within 7 μm sections of CNS tissue isolated from vehicle treated (left) and CBD treated (right) EAE mice (Green = CCR2, Red = actin, Blue = cell nuclei)