FIG. 3.

Assays of HDV replication in avian and mammalian cells during reduced expression of small delta protein. Cultures of LMH (lanes 1 to 5) or Huh7 cells (lanes 6 to 10) were cotransfected with fixed amounts of pGG119 and pJC110 along with various amounts of pTW198. The ratios of pTW198 to pJC110 are as follows: lanes 1 and 6, 0:1; lanes 2 and 7, 1:1; lanes 3 and 8, 0.3:1; lanes 4 and 9, 0.03:1; lanes 5 and 10, untransfected. At 4 days after transfection, RNA and protein were extracted. RNA was analyzed by Northern blot for antigenomic RNA (A) or genomic RNA (B). Protein was assayed by immunoblot for delta protein (C).