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Figure 2. TMEM147-AS1 deficient exerts repressing activity on GC cell phenotype. (A) qRT-PCR was utilized for the measurement of TMEM147-AS1 in GC cells when were transfected with si-TMEM147-AS1 or si-NC. ***p < 0.001 (n = 3) compared with si-NC. (B) CCK-8 assay was conducted in GC cells after TMEM147-AS1 depletion. ***p < 0.001 (n = 3) compared with si-NC. **p < 0.01 (n = 3) compared with si-NC. (C) The colony formation of si-TMEM147-AS1-expressing GC cells was quantified via colony formation assay. ***p < 0.001 (n = 3) compared with si-NC.