Figure 9. MiR-326 inhibitor reintroduction counteracts the effects of si-TMEM147-AS1 in GC cells. (A) qRT-PCR verified the decrease of miR-326 by miR-326 inhibitor. ***p < 0.001 (n = 3) compared with NC inhibitor. (B) Cell proliferation was examined in GC cells which were transfected with si-NC, si-TMEM147-AS1+miR-326 inhibitor, or si-TMEM147-AS1+NC inhibitor. **p < 0.01 (n = 3) compared with si-NC. ^##p < 0.01 (n = 3) compared with si-TMEM147-AS1+NC inhibitor. ^#p < 0.05 (n = 3) compared with si-TMEM147-AS1+NC inhibitor. (C) The colony formation was confirmed in TMEM147-AS1-silenced GC cells which were further received miR-326 inhibitor or NC inhibitor reintroduction. ***p < 0.001 (n = 3) compared with si-NC. ^###p < 0.001 (n = 3) compared with si-TMEM147-AS1+NC inhibitor. (D) GC cells were transfected with si-NC, si-TMEM147-AS1+NC inhibitor or si-TMEM147-AS1+miR-326 inhibitor. The migratory and invasive capacities of GC cells were examined. ***p < 0.001 (n = 3) compared with si-NC. ^###p < 0.001 (n = 3) compared with si-TMEM147-AS1+NC inhibitor. ^##p < 0.01 (n = 3) compared with si-TMEM147-AS1+NC inhibitor.