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. 2023 May 5;34(6):ar63. doi: 10.1091/mbc.E22-07-0296

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© 2023 Mercadante et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — Oscillatory redistribution of cortical dynein activity in computational modeling enhances centrosome clustering. (A–C) Top, Traces of centrosome movement over time from a simulation with dynein-enriched regions (with = 0.5) oscillating between the upper-right (0 to π/2, solid pink arc) and the lower-left (−π to −π/2, dashed pink arc) quadrants with periods of t = 5 min (A), 10 min (B), or 15 min (C). Initial centrosome position indicated by a red “x,” and final centrosome position indicated by a blue asterisk. Grayscale indicates time. (A–C) Bottom, Heat map representing the pairwise distances between all centrosomes from the corresponding simulation above. Black dotted lines indicate a time point when the quadrant of cortical dynein enrichment is updated. (D) Plots depicting the magnitude of cortical dynein–derived forces over time (blue bars, scale on left axis) and the distance between each centrosome and the midpoint of dynein localization (red trace, scale on right axis) from the simulation shown in C, where cortical dynein is redistributed at t = 15 min. (E) Quantification of transient bipolar spindle formation (each of the two poles having one or more clustered centrosomes) for simulations with either three or four centrosomes. Data are for 30 simulations.

Inline graphic — Oscillatory redistribution of cortical dynein activity in computational modeling enhances centrosome clustering. (A–C) Top, Traces of centrosome movement over time from a simulation with dynein-enriched regions (with = 0.5) oscillating between the upper-right (0 to π/2, solid pink arc) and the lower-left (−π to −π/2, dashed pink arc) quadrants with periods of t = 5 min (A), 10 min (B), or 15 min (C). Initial centrosome position indicated by a red “x,” and final centrosome position indicated by a blue asterisk. Grayscale indicates time. (A–C) Bottom, Heat map representing the pairwise distances between all centrosomes from the corresponding simulation above. Black dotted lines indicate a time point when the quadrant of cortical dynein enrichment is updated. (D) Plots depicting the magnitude of cortical dynein–derived forces over time (blue bars, scale on left axis) and the distance between each centrosome and the midpoint of dynein localization (red trace, scale on right axis) from the simulation shown in C, where cortical dynein is redistributed at t = 15 min. (E) Quantification of transient bipolar spindle formation (each of the two poles having one or more clustered centrosomes) for simulations with either three or four centrosomes. Data are for 30 simulations.