FIG. 1.

Full-length infectious cDNA clone of MVE-1-51. Plasmid pM212 contains the full-length cDNA of MVE-1-51 (11,013 nucleotides), which includes an open reading frame (ORF) encoding a polyprotein of 3,434 amino acids (solid line). Restriction sites used in subcloning and genetic manipulations are shown (numbering is from the 5′-terminal nucleotide in MVE-1-51). Sequences at the 5′ (plus an ApaI site and T7 promoter sequence) and 3′ (plus a NotI site) termini of the viral genome are shown (boxed). The vector pBR322* is a deleted version of pBR322 (see Materials and Methods). The ampicillin resistance gene (Ap), the ROP gene, and the origin of DNA replication (ORI) are shown. NCR, noncoding region.