Movie S2.

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Effect of limited number of MTs that can connect to a KT on final CH position and orientation: This movie shows the first 5 min of cell dynamics and is related to Figure 6, B and E in the main text. The cell includes one CH with arms (blue); each centrosome has 1,500 MTs (green). The KT surface area is 0.5 μm² (orange). The pushing/pulling force per MT is 10 pN/10 pN, and the KT-MT dissociation rate depends on the phosphorylation degree of the Ndc80 linkers (see Table S2). The maximum of three MTs can be attached to a KT at the same time. A CH is shifted to one of the poles and the centromere axis is perpendicular to the vector connecting this pole to the centromere center (Figure 2D in the main text). The duration of the movie is 9 s (the movie is played ~30 times faster than the process).