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. 2023 May 5;34(6):ar57. doi: 10.1091/mbc.E22-11-0507

Movie S3.

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Crowding effect: The movie shows 15 min of cell dynamics and is related to Figure 7 in the main text. The cell includes five CH with arms (blue) and centrosomes each having 1,500 MTs (green). The KT surface area is 0.5 μm2 (orange). The pushing/pulling force per MT is 10 pN/10 pN, and the KT-MT dissociation rate depends on the phosphorylation degree of the Ndc80 linkers (see Table S2). The maximum of three MTs can be attached to a KT at the same time. All five CHs are randomly placed and oriented within the area between the two poles. The duration of the movie is 30 s (the movie is played ∼30 times faster than the process).