Table 2.

Top 15 differentially methylated CpG sites by tobacco consumption.

CpG	chr	position	Gene Symbol	Gene Group	Less than 1 per week	Less than 1 per day	1–5 per day	6–10 per day	11–20 per day	More than 20 per day	F	P-Value	Adjusted P-Value	EWAS catalogue (same direction/total studies)
cg05575921	chr5	373378	AHRR	Body	−0.028	−0.054	−0.097	−0.216	−0.183	−0.217	208.2	1.3E–226	9.7E–221	26/26
cg21566642	chr2	233284661			−0.030	−0.058	−0.066	−0.109	−0.111	−0.160	59.7	1.6E–70	5.9E–65	22/22
cg01940273	chr2	233284934			−0.016	−0.034	−0.045	−0.066	−0.069	−0.109	53.6	2.4E–63	5.9E–58	22/22
cg03636183	chr19	17000585	F2RL3	Body	−0.007	−0.017	−0.029	−0.064	−0.053	−0.094	50.9	2.8E–60	5.2E–55	21/21
ch.6.2768623F	chr6	144037285	PHACTR2	Body	−0.002	−0.001	0.000	0.009	−0.005	0.350	49.1	4.7E–58	7.0E–53	Not reported
cg21161138	chr5	399360	AHRR	Body	−0.008	−0.016	−0.015	−0.071	−0.043	−0.069	40.6	5.7E–48	7.1E–43	16/16
cg17739917	chr17	38477572	RARA	5‘UTR	−0.008	−0.053	−0.034	−0.069	−0.082	−0.116	38.2	4.5E–45	4.8E–40	7/7
cg15928392	chr2	81694446			0.000	0.000	0.001	−0.001	−0.002	−0.106	34.1	3.4E–40	3.2E–35	Not reported
cg16774290	chr7	4824628	AP5Z1	Body	0.000	0.000	0.000	−0.001	0.000	−0.100	33.2	4.1E–39	3.4E–34	Not reported
cg11173636	chr10	65632259			−0.008	0.017	0.024	0.018	0.339	−0.020	30.5	8.5E–36	6.3E–31	Not reported
cg09935388	chr1	92947588	GFI1	Body	−0.018	−0.030	−0.029	−0.100	−0.069	−0.134	30.0	3.5E–35	2.4E–30	20/20
cg26703534	chr5	377358	AHRR	Body	−0.004	−0.013	−0.016	−0.052	−0.034	−0.049	29.9	4.0E–35	2.4E–30	14/14
cg14990808	chr6	28493651	GPX5	TSS200	−0.002	−0.022	0.005	0.002	0.001	0.246	26.3	1.1E–30	6.2E–26	Not reported
cg25648203	chr5	395444	AHRR	Body	−0.006	−0.005	−0.011	−0.047	−0.037	−0.057	24.8	6.2E–29	3.3E–24	18/18
cg04176674	chr14	21121564			−0.004	−0.002	0.001	0.005	0.000	0.343	24.7	7.8E–29	3.9E–24	Not reported

Note: The CpG sites are annotated based on the chromosome (chr), the position (pos), the gene symbol from HGNC, and the gene group (based on the position of the CpG regarding the nearest gene). Each CpG site has a beta value for each consumer group vs non-consumers, a F-statistic (F), a nominal p-value, and an adjusted p-value by Bonferroni. The last column represents the number of studies in the EWAS catalogue that reported the same effect direction compared to the total studies that reported the beta value for the specific CpG site.