Table 3.
Top 15 differentially methylated CpG sites by alcohol consumption.
| CpG | chr | position | Gene Symbol | Gene Group | On special occasions | Once per week | 3–5 times per week | Regularly | F | P-Value | Adjusted P-Value | Lohof et al (direction) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| cg06690548 | chr4 | 139162808 | SLC7A11 | Body | −0.003 | −0.005 | −0.015 | −0.031 | 103.1 | 5.9E–83 | 4.4E–77 | – |
| cg11376147 | chr11 | 57261198 | SLC43A1 | Body | −0.001 | −0.002 | −0.006 | −0.010 | 49.3 | 2.4E–40 | 8.9E–35 | – |
| cg26457483 | chr1 | 120256112 | PHGDH | Body | −0.001 | −0.004 | −0.017 | −0.028 | 39.7 | 1.5E–32 | 3.8E–27 | – |
| cg14476101 | chr1 | 120255992 | PHGDH | Body | −0.002 | −0.005 | −0.016 | −0.031 | 36.2 | 1.1E–29 | 2.0E–24 | – |
| cg18120259 | chr6 | 43894639 | LINC01512 | Body | −0.002 | −0.004 | −0.009 | −0.019 | 35.0 | 1.1E–28 | 1.6E–23 | – |
| cg06088069 | chr14 | 75895604 | JDP2 | 5‘UTR | −0.002 | −0.003 | −0.007 | −0.013 | 34.2 | 5.0E–28 | 6.1E–23 | – |
| cg21998542 | chr10 | 33605101 | NRP1 | Body | −0.002 | −0.005 | −0.009 | −0.019 | 29.0 | 9.2E–24 | 9.8E–19 | – |
| cg15837522 | chr8 | 117892654 | −0.003 | −0.011 | −0.022 | −0.031 | 28.7 | 1.7E–23 | 1.6E–18 | – | ||
| cg06644515 | chr1 | 173834831 |
SNORD47;GAS5; SNORD81;SNORD80; SNORD78;SNORD79 |
TSS1500;Body; TSS1500;TSS1500; TSS200;TSS1500 |
−0.001 | −0.001 | −0.007 | −0.013 | 27.6 | 1.3E–22 | 1.1E–17 | – |
| cg12116137 | chr17 | 1576449 | PRPF8 | Body | 0.001 | 0.001 | 0.008 | 0.018 | 25.5 | 7.5E–21 | 5.5E–16 | ++ |
| cg19693031 | chr1 | 145441552 | TXNIP | 3‘UTR | −0.004 | −0.003 | −0.011 | −0.026 | 25.1 | 1.8E–20 | 1.2E–15 | – |
| cg12825509 | chr3 | 185648568 | TRA2B | Body | 0.000 | −0.004 | −0.010 | −0.016 | 24.6 | 4.5E–20 | 2.8E–15 | – |
| cg14346162 | chr21 | 44490229 | CBS | Body | 0.001 | 0.004 | 0.009 | 0.014 | 23.5 | 3.1E–19 | 1.8E–14 | Not reported |
| cg12973487 | chr19 | 1623075 | TCF3 | Body | 0.004 | 0.005 | 0.007 | 0.014 | 22.6 | 2.0E–18 | 1.0E–13 | ++ |
| cg05713943 | chr13 | 97912352 | MBNL2 | 5‘UTR | 0.001 | 0.000 | −0.005 | −0.008 | 21.8 | 8.7E–18 | 4.2E–13 | – |
Note: The CpG sites are annotated based on the chromosome (chr), the position (pos), the gene symbol from HGNC, and the gene group (based on the position of the CpG regarding the nearest gene). Each CpG site has a beta value for each consumer group vs non-consumers, a F-statistic (F), a nominal p-value, and an adjusted p-value by Bonferroni. We also included one column with the direction of the effect in a previous study (Lohoff et al (31)) for those that were significant in their analysis.