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. 2023 May 23;45(1):2212079. doi: 10.1080/0886022X.2023.2212079

Figure 1.

Figure 1.

The characteristics of PMSCs and BMSCs. (a) The cell numbers of PMSCs (n = 6) and BMSCs (n = 6) during the three-day cell culture. (b) The expressions of CFSE of PMSCs and BMSCs collected after the three-day cell culture and the 2nd three-day cultivation were detected by flow cytometry. (c) The expressions of CFSE of PMSCs and BMSCs collected after the 2nd three-day cultivation were compared by using flow cytometry. (d) The expressions of Sca-1, CD29, CD44, CD31, and CD117 on the surfaces of PMSCs collected after the 2nd three-day culture were detected by flow cytometry. (e) The expressions of surface marks of BMSCs collected after the 2nd three-day culture were detected by flow cytometry. (f) The osteogenic differentiation of PMSCs and BMSCs was identified on the 21th day by Alizarin Red staining. Scale bars: 100 μm. (g) The adipogenic differentiation of PMSCs and BMSCs was identified on the 21th day by Oil Red O staining. Scale bars: 100 μm. (h) The surface marks of PMSCs and BMSCs collected after the 2nd three-day cultivation were compared by using flow cytometry. An independent-sample t-test evaluated differences for between-group comparisons. *p < .05.