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. 2023 May 23;45(1):2212079. doi: 10.1080/0886022X.2023.2212079

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — PMSCs promoted Treg increase and differentiation. (a) Immunofluorescence staining of Foxp3 (green) and DAPI (blue) in the kidney. Scale bars: 100 μm. (b) Helper T cells in the kidney were demonstrated through the expression of CD45 and CD4. Tregs were verified from helper T cells through the expression of CD25 and Foxp3. The statistical analysis of Treg proportions in helper T cells from the kidney single-cell suspension in the NC (n = 5), IR (n = 5), IR + BMSC (n = 5), and IR + PMSC (n = 5) groups. (c) Immunofluorescence staining of Foxp3 (green) and DAPI (blue) in the spleen. Scale bars: 100 μm. (d) Detection of CD45⁺CD4⁺CD25⁺Foxp3⁺Tregs from the spleen single cell suspension in the NC, IR, IR + BMSC, and IR + PMSC groups by flow cytometry. The statistical analysis of Treg proportions in helper T cells from the spleen in the NC (n = 5), IR (n = 5), IR + BMSC (n = 5), and IR + PMSC (n = 5) groups. (e) The statistical analysis of Treg proportions in CD4⁺ T cells from the kidney single-cell suspension in the normal spleen (n = 4), splenectomy (n = 4), splenectomy + BMSC (n = 4), and splenectomy + PMSC (n = 4) groups. (f) The statistical analysis of CD4⁺CD25⁺Foxp3⁺ Treg proportions in CD4⁺ T cells collected after in vitro culture in the CON (n = 3), BMSC (n = 3), and PMSC (n = 3) groups. The Kruskal–Wallis one-way analysis of variance and post hoc tests with all pairwise were used to evaluate the differences. *p < .05; **p < .01; ***p < .001.