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. 2023 May 23;45(1):2212079. doi: 10.1080/0886022X.2023.2212079

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — PMSCs inhibited inflammation through Tregs. The IR kidney and spleen in the NC, IR + IgG, IR + PC61, IR + PC61 + BMSC, and IR + IgG + PMSC groups were harvested on the day 2 after operation. (a, b) Immunofluorescence staining of Foxp3 (green) and DAPI (blue) in the kidney (a) and spleen (b). Scale bars: 100 μm. (c) Detection of CD45⁺CD4⁺CD25⁺Foxp3⁺Tregs from the spleen and kidney single cell suspensions using flow cytometry. The levels of IL-6 (d), IFN-γ (e), and IL-10 (f) in IR kidneys, as determined by ELISA. (g) Immunofluorescence staining of F4/80 (red) and DAPI (blue) in IR kidneys. Scale bars: 100 μm. The Kruskal–Wallis one-way analysis of variance and post hoc tests with all pairwise were used to evaluate the differences. ns, p > .05; *p < .05; **p < .01.