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. 2023 May 23;45(1):2212079. doi: 10.1080/0886022X.2023.2212079

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 7. — mTOR exerted as a critical mediator during PMSC promotion for Tregs. (a) The statistical analysis of mTOR phosphorylation levels in CD4⁺CD25⁺Foxp3⁺Treg collected after in vitro culture in the CON (n = 3) and PMSC (n = 3) groups. (b) The gating strategy to determine mTOR phosphorylation in Tregs from spleen single-cell suspension. The statistical analysis of Treg proportions in helper T cells and their mTOR phosphorylation level from Tregs in the NC (n = 5), IR (n = 5), and IR + PMSC (n = 5) groups. (c) Detection of CD4⁺CD25⁺Foxp3⁺Tregs from the kidney single cell suspension in the NC, IR, and IR + PMSC groups by flow cytometry. The statistical analysis of Treg proportions in helper T cells and their mTOR phosphorylation level from Tregs in the NC (n = 5), IR (n = 5), and IR + PMSC (n = 5) groups. (d) The gating strategy to determine mTOR phosphorylation in Tregs from kidney single cell suspension. The statistical analysis of Treg proportions in helper T cells and their mTOR phosphorylation level from Tregs in the NC (n = 5), IR (n = 5), mTOR^–/–IR (n = 5), mTOR^–/–IR + PMSC (n = 5), and IR + PMSC (n = 5) groups. (e) Detection of CD4⁺CD25⁺Foxp3⁺Tregs from the spleen single cell suspension in the NC, IR, mTOR^–/–IR, mTOR^–/–IR + PMSC, and IR + PMSC groups by flow cytometry. The statistical analysis of Treg proportions in helper T cells and their mTOR phosphorylation level from Tregs in the NC (n = 5), IR (n = 5), mTOR^–/–IR (n = 5), mTOR^–/–IR + PMSC (n = 5), and IR + PMSC (n = 5) groups. (f) The statistical analysis of Treg proportions in helper T cells collected after in vitro culture in the CON (n = 4) and PMSC (n = 4) groups. The differences for between-group comparisons were analyzed by an independent-sample t-test. The differences for among-group comparisons were analyzed by the Kruskal–Wallis one-way analysis of variance and post hoc tests with all pairwise. ns, p > .05; *p < .05; **p < .01; ***p < .001.