Fig. 5.

(a–c) Relative gene expression of Il6 (a), Sele (b) and Edn1 (c) from whole islets isolated from NT and TG mice after 1 year on LF or HF. Values are shown as fold change relative to the LF-fed NT group. (d–j) Representative images of HF-fed NT (d, f) and HF-fed TG (e, g) mouse pancreas sections stained with anti-VCAM-1 (d, e; brown) or anti-CD31 (f, g; brown) together with counterstaining with haematoxylin (d, e, f, g; blue) and/or Thioflavin S (f, g; green). The dashed lines denote islet boundary; red arrows denote positive VCAM-1 staining. Scale bars, 100 μm. Mean islet area (h), percentage of CD31-positive area per islet area (i) and capillary density (j) of the pancreas sections were determined. n=5–7 mice/group; *p<0.05, **p<0.01 and ***p<0.001