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IL-6-like factor activity in culture supernatants at 5 and 7 days p.i. (5D and 7D PI), measured by the proliferation of IL-6-dependent B9 cells. Cell proliferation was measured by MTT incorporation assessed as the OD at 570 nm. The results represent three separate experiments (mean OD ± SD). Recombinant human IL-6 (rhIL-6; 10 U/ml) and CM were included as positive controls. Asterisks indicate significant differences between virus-free and IBDV-infected groups (P < 0.05).
