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. 2023 May 24;18(5):e0285905. doi: 10.1371/journal.pone.0285905

Fig 6. Heatmap comparing the metabolites extracted from feces of NOD mice at different times point pre- and post-vaccination using GC-MS.

Fig 6

Feces collected from mice before and after vaccination at days 3, 7, 14, and 30. The amount represents the relative percentage of a metabolite in relation to total metabolites detected in a fecal pellet. Metabolite average relative percentages of 5 mice at each time point are displayed as blue bars. The statistical significance was calculated using multiple paired t test with Benjamini, Kreiger, and Yekutiele corrected significance level indicated asterisks (*p < 0.05, **p<0.01, ***p<0.005, ****p<0.001). Red asterisks mean a significant increase and black asterisks mean a significant decrease in comparison to the pre-treated group. SCFA (short-chine fatty acid); SFA (saturated fatty acid); UFA (unsaturated fatty acid); MUFA (monounsaturated fatty acid); PUFA (polyunsaturated fatty acid); TCA (tricarboxylic acid cycle); nd (not detected).