FIG. 1.

Effects of HIV-1 Ba-L replication on iNOS mRNA transcription and NO₂⁻ production in human MDM cultures. (A) ●, level of HIV replication determined by the measure of RT activity in culture supernatants (mean of three independent culture wells ± SD); ○, RT activity in uninfected controls (mean of three independent culture wells ± SD); ■, production of nitrites in culture supernatants of infected macrophages (mean of three independent culture wells ± SD); □, nitrite production in uninfected control cultures (mean of three independent culture wells ± SD). (B) Expression of iNOS mRNA in the same culture of infected, or uninfected macrophages, determined as the ratio of the signal obtained for tested mRNA to the signal obtained for GAPDH mRNA (mean of two independent culture wells). Bars represent the mean of two independent measures.