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. Author manuscript; available in PMC: 2023 May 24.
Published in final edited form as: Cell Rep. 2023 Feb 28;42(3):112166. doi: 10.1016/j.celrep.2023.112166

Figure 7. Loss of Tcf21 accelerates Tcf21 LC developmental progress.

Figure 7.

(A) Tcf21 LCs isolated from WT and KO mice at P18, 5 weeks, 1 week HFD (WT only), and 12 weeks HFD (WT only) were subjected to scRNA-seq. The UMAP graph shows the clustering of Tcf21 LCs from different sample groups, which include 7,260 WT cells, 122 KO cells at P18, and 555 KO cells at 5 weeks.

(B and C) Violin plots show the expression of Tcf21 (B) and Dlk1 (C) in Tcf21 LCs of different samples.

(D and E) Monocle 2 pseudotime analysis of WT and KO Tcf21 LCs at P18.

(F) RNA velocity analysis of WT and KO Tcf21 LCs at P18.

(G) A UMAP graph shows the clustering of Tcf21 LCs of WT and KO 5-week mice and WT 1 week HFD mice.

(H and J) Monocle 2 pseudotime analysis of Tcf21 LCs of WT and KO 5-week mice and WT 1 week HFD mice.

(K and L) RNA velocity analysis of Tcf21 LCs of WT and KO 5-week mice and WT 1 week HFD mice (K) and a UMAP showing the expression of Dlk1 on the RNA velocity trajectory (L).

See also Figures S6 and S7.