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. 2023 May 11;12:e85041. doi: 10.7554/eLife.85041

Figure 3. Age dependence of retro-Tango.

The retro-Tango signal is observed in 5 day intervals upon ligand expression in the EPGs. The signal accumulates with time and saturates around day 10 post-eclosion. Males were analyzed for all panels. Presynaptic mtdTomato (magenta) and neuropil (grey). Scale bars, 50 μm.

Figure 3.

Figure 3—figure supplement 1. Quantification of the pixel intensity for the signal of retro-Tango when initiated from the EPG neurons.

Figure 3—figure supplement 1.

Comparison of the pixel intensities in the central complex for the presynaptic signal in males of different ages where the retro-Tango was initiated from the EPG neurons (n=5 brains each). Dots represent data points, the horizonal lines represent the mean and the error bars represent the standard error of the mean. One-way ANOVA, *: p<0.05, ns: not significant.
Figure 3—figure supplement 2. Age dependence of the retro-Tango signal in the presynaptic partners of the GFs in males.

Figure 3—figure supplement 2.

(A) retro-Tango signal is observed in 5-day intervals upon ligand expression in the GFs. The signal accumulates over time. Males were analyzed for all panels. Presynaptic mtdTomato (magenta) and neuropil (grey). Scale bars, 50 μm. (B) Comparison of the pixel intensities in the whole brain for the presynaptic signal in males of different ages where the retro-Tango was initiated from the GF neurons (n=10 hemibrains each). Dots represent data points, the horizonal lines represent the mean and the error bars represent the standard error of the mean. One-way ANOVA, **: p<0.01, ns: not significant.
Figure 3—figure supplement 3. Age dependence of the retro-Tango signal in the presynaptic partners of the GFs in females heterozygous for the reporter.

Figure 3—figure supplement 3.

(A) retro-Tango signal is observed in 5-day intervals upon ligand expression in the GFs. The signal does not seem to change significantly over time. Females heterozygous for the reporter were analyzed for all panels. Presynaptic mtdTomato (magenta) and neuropil (grey). Scale bars, 50 μm. (B) Comparison of the pixel intensities in the whole brain for the presynaptic signal in females of different ages where the retro-Tango was initiated from the GF neurons (n=10 hemibrains each). Dots represent data points, the horizonal lines represent the mean and the error bars represent the standard error of the mean. One-way ANOVA, ns: not significant.