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. 2023 May 11;12:e85041. doi: 10.7554/eLife.85041

Figure 5. Assessing the specificity of retro-Tango in a sexually dimorphic circuit.

Figure 5.

(A) Initiating retro-Tango in aDNs in male flies reveals visual projection neurons (arrow) as presynaptic partners (223±59 neurons in 5 brains). (B) Initiating retro-Tango in aDNs in females results in presynaptic reporter expression in the lateral antennal lobe tract (arrowhead), the SEZ (asterisk), and the LH (hash) (24±11 neurons in 5 brains). 15do males hemizygous for the tdTomato reporter (A) and females heterozygous for the reporter (B) were analyzed. Postsynaptic GFP (cyan), presynaptic mtdTomato (magenta) and neuropil (grey). Scale bars, 50 μm.